The Acropolis did not change very much after the Romans had conquered it in 86 BC. During the Hellenistic period a four-in-hand was placed in front of the Propylaea to honour king Eumenes II who was a benefactor of the city. The Romans did built a small round temple for the cultus of emperor Augustus. However, the Romans did adept the building to the look of the Acropolis as it had a collonade with nine Ionic columns. Now the new Roman gods had settled themselved between the older Greek gods who continued to live on.

The Romans had learned a lot of the Greek civilisation, so in return they respected the traditional importance of the Acropolis. Around 50 AC emperor Claudius even tried to create a better access by building a monumental stairway to the Propylaea. Furthermore the famous monument of Agrippa, the general of Augustus, was placed outside the Acropolis. Unfortunately the only thing that remains of this impressive statue is the socle.

Other buildings which were erected by the Romans where the Stoici of Eumenes and the massive Odeion which was financed by Herodes Atticus, an incredibly wealthy landowner from Marathon. The Odeion was built around 161 AC and marble seats and a magnificent roof made out of cedarwood.